A Molecular‐Splicing Strategy for Constructing a Near‐Infrared Fluorescent Probe for UDP‐Glucuronosyltransferase 1A1

UDP-glucuronosyltransferase 1A1 (UGT1A1) is a vital metabolic enzyme responsible for the clearance of endogenous substances and drugs. Hitherto, development fluorescent probes UGTs was severely restricted due to poor isoform selectivity on–off or blue-shifted fluorescence response. Herein, we established novel “molecular-splicing” strategy construct highly selective near-infrared (NIR) probe, HHC, UGT1A1, which exhibited NIR signal at 720 nm after UGT1A1 metabolism. HHC then successfully used real-time imaging in living cells animals monitor bile excretion function. In summary, an isoform-specific probe has been developed monitoring activity systems, high-throughput screening inhibitors visual evaluation